Research work · 1985
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Gérard I. Evan, George K. Lewis, Gary M. Ramsay
Molecular and Cellular Biology
Abstract
Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also recognize the mouse c-myc gene product. None of the antibodies sees the chicken p110gag-myc protein. All six antibodies recognize immunoblotted p62c-myc. These reagents also provide the basis for an immunoblotting assay by which to quantitate p62c-myc in cells.