Research work · 1976
Multiplication of a Human Parasite ( Leishmania donovani ) in Phagolysosomes of Hamster Macrophages in Vitro
Kwang Poo Chang, Dennis M. Dwyer
Science
Abstract
Leishmania donovani, the etiological agent of human visceral leishmaniasis, was grown in hamster peritoneal macrophages in vitro. By electron microscopy, using a lysosomal marker, these parasitic protozoa were seen to multiply within host cell phagolysosomes. The survival mechanism of this intracellular parasite is based apparently upon resistance to macrophage lysosomal enzymic digestion.