Research work · 1991
Expression Cloning of an Adenylate Cyclase-Coupled Calcitonin Receptor
Herbert Yih-Fuu Lin, Tonja L. Harris, Merilee S. Flannery, Alejandro A. Aruffo, Eugene H. Kaji, Alan H. Gorn, Lee F. Kolakowski, Harvey F. Lodish, Steven R. Goldring
Science
Abstract
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd approximately 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.